| 1. | Methods and applications of inserting a stop codon into a cloned gene with pcr 在已克隆基因末端添加终止密码的方法与应用 |
| 2. | Using 3 ' race the transcription stop site mapped 27nt downstream of the putative translation stop codon 3 ’ race分析结果表明转录的终止位点在翻译终止密码子下游的27个碱基处。 |
| 3. | The fact results in the conclusion that the bases related to the stop codon tga are strictly constrained to be used 由此得出结论:终止密码子对蛋白质编码区碱基的使用具有限制作用。 |
| 4. | The statistical results show that the bases and dinucleotides with low contents are just associated with those in the stop codon tga 对单核苷酸和双核苷酸的使用进行统计分析,结果表明与终止密码子对应的组分的含量都很低。 |
| 5. | Rt - pcr were performed to obtain the information of transcriptional profile , the transcription stop site was mapped to 4bp downstream of stop codon tga by 3 ' race 对ha99基因作了相关的计算机分析,用rt - pcr方法从mrna水平上对该基因进行了鉴定,证明是一个晚期基因。 |
| 6. | A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon 在其起始密码子上游- 65nt发现杆状病毒晚期启动子转录起始信号ataag ,在其终止密码子下游- 14nt发现polya加尾信号aataaa 。 |
| 7. | The start reading framae and stop codons , base composition in protein - coding genes and the codon usage of amino acids in scolopendra multilane were compared with the three other myriapods 本研究在蛋白质编码基因起始阅读框和终止密码子、蛋白质编码区的碱基组中文摘要成、氨基酸及密码子的利用等方面把少棘蜈蚣与另三种多足类进行了比较。 |
| 8. | Pcr method was used to identify candidate ms 188 . sequence analysis indicated that a point mutation occurred in the second exon of the atmyb103 gene in male sterile mutant with caa ( gln ) replaced by a stop codon taa 利用pcr的方法从突变体中扩增atmyb103基因并进行序列分析,结果表明突变体中atmyb103基因第二个外显子上发生了点突变,由原来编码谷氨酰胺的caa密码子突变为终止密码子taa 。 |
| 9. | A late transcriptional motif , ataag , was found at - 50nt upstream of the translational start codon atg , while two tata box was located at - 112nt and - 189nt upstream of atg . a typical poly ( a ) signal was found at 12nt downstream of the translational stop codon 在hel起始密码子atg上游50位有强晚期启动子转录起始信号ataag ,在? 112位和? 189位存在两个tatabox ,但未发现早期转录信号cagt 。 |
| 10. | Vp2 and vp3 . the genes , encoding them , were located in a same orf , and they possess of common stop codons . furthermore , amino acid sequence of vpl contained the fu1l sequence of vp2 and vp3 , so vp1 gene become interest gene . in this research Gpv的三种结构蛋白vp1 、 vp2 、 vp3位于同一开放阅读框内,共用同一终止密码子, vp1包含了组成vp2 、 vp3的所有氨基酸,因其具有这一特殊性,成为本研究的目的基因。 |